Opracowanie algorytmów do analizy zdjęć z mikroskopii fluorescencyjnej
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2017-02
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Institute of Nuclear Physics Polish Academy of Sciences
Abstract
The  fluorescence  microscopy  is  one  of  the  fastest  techniques  of  DNA  damage  visualisation induced  by  physical  or  chemical  factors.  For  this  reason,  the  area  of  algorithms  for  images analysis    from    fluorescence    microscopy    continually    develops. This    report    contains presentation of algorithms elaborated for the assessment of relative amount of DNA damage, visualised with the fluorescent dyes Alexa Fluor 488 and DAPI. Methods of the determination of the noise level and signal to noise ratio in the images, as well as methods of noise reduction and image quality optimization have been presented.
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Sponsorship:
The Małopolska Regional Operational Programme Measure 5.1 Krakow Metropolitan Area as an important hub of the European Research Area for 2007- 2013, project No. MRPO.05.01.00-12-013/15