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Browsing by Author "Miszczyk, Justyna"

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    Influence of therapeutic proton beam on glioblastoma multiforme proliferation index – A preliminary study
    (Acta Physica Polonica A, 2019) Bogdali-Suślik, Anna M.; Rawojć, K.; Miszczyk, Justyna; Panek, Agnieszka; Woźniak, Monika; Szewczyk, Katarzyna; Książek, Teofila; Bik-Multanowski, Mirosław; Swakoń, Jan; Komenda, Wiktor; Mojżeszek, Natalia; Kajdrowicz, Tomasz; Kopeć, Renata; Olko, Paweł
    The goal of the presented study was to compare the proliferation of different U118 MG and U251 MG glioblastoma cell lines irradiated with proton beam or X-rays in dose range 0.5-10.0 Gy. Cytokinesis-block micronucleus (CBMN) assay was carried out to study changes in proliferation presented as nuclear division index (NDI). Preliminary results suggest that protons and X-rays influence GBM (glioblastoma multiforme) cellular proliferation differently. Therapeutically, a decrease in NDI values with the increase in both types of radiation dose was found only for U251 MG cell line, thus can be classified as more radiosensitive than U118 MG cell line. Also for U251 MG GBM cell line, a therapeutic proton beam was more effective in inhibition of proliferation than X-rays. Genetic differences between GBM are supposed to be involved in the increased radiosensitivity, which is planned to be studied further by genes expression analysis.
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    Multimodal approach for ionizing radiation damage investigation
    (Institute of Nuclear Physics Polish Academy of Sciences, 2015) Kwiatek, Wojciech M.; Adamczyk, Jolanta; Bobrowska, Justyna; Borkowska, Anna; Lekka, Małgorzata; Lekki, Janusz; Lipiec, Ewelina; Miszczyk, Justyna; Pabijan, Joanna; Paluszkiewicz, Czesława; Panek, Agnieszka; Piergies, Natalia; Pogoda, Katarzyna; Prauzner-Bechcicki, Szymon; Quaroni, Luca; Roman, Maciej; Wiecheć, Anna; Wiltowska-Zuber, Joanna
    The new, leading edge laboratory has been established and put into routine operation. The laboratory is focused on multimodal studies of the cellular response to ionizing radiation and provides support and research facilities for the Bronowice Cyclotron Centre. Spectroscopic imaging of cells and tissues (examining in particular the internal structure of cells, the cytoskeleton organization, cells’ mechanical and biochemical properties), as well as research at the molecular level, has been applied in research for the purpose of searching new strategies of prevention and therapies of cancer and other pathologies and in fundamental research in the field of structural and vibrational analysis of condensed matter. The multimodal approach, illustrating the full laboratory potential, has been applied to radiation effect studies of transitional cells – human bladder carcinoma cells (T24 cell line). Cells were irradiated with three X-ray radiation doses: 1 Gy, 3 Gy, and 10 Gy and subsequently studied using all instruments of the new laboratory. The presented results demonstrated that the AFM elasticity measurements can be applied to quantitatively estimate alterations induced upon Xray irradiation at the single cell level. A combination of AFM and InfraRed Spectroscopy (NanoIR2 setup) was successful in characterization molecular changes occurring in the nuclear environment following cellular irradiation. Particularly useful information acquired was the observation of changes in distribution of macromolecules with a spatial resolution at the level of the single organelle. The observed changes correlate with radiation dose and thus may become a tool for studying the biological effects of radiation exposure. The application of Raman microspectroscopy for radiation-induced damage investigations provided detection of such spectral changes as strand breaks, base unstacking, and DNA conformational transformations. These studies give crucial information about the damage associated with irradiation and cellular response for radiation dose at single cell level. Moreover, Raman spectroscopy provides possibility to investigate structural changes present within the same sample. Complementary UV–VIS technique, on the basis of γ-H2AX test, delivered quantitative data of radiation damage, manifesting in presence of double strand breaks in DNA in 1st and 2nd day of culture (1 hour and 24 hours after irradiation).