# README: Study Data Documentation
## 1. Overview
This README file provides a detailed explanation of the samples, methodologies, and data processing techniques applied during the course of the study. The information herein is essential for understanding the context and structure of the data uploaded to the repository.
## 2. Samples
- **Sample Type:** Metallic nanoparticles (gold)
- **Sample Collection Method:** Synthesized via chemical reduction method, modified by electrostatic interaction by LbL (PEI, PEI/PSS)  and by using chitosan solution 
- **Number of Samples:** 50 distinct nanoparticle batches
- **Storage and Handling:** Stored at 4C in aqueous solution to sustain the stability 
- **Additional Metadata:** Samples labeled with unique identifiers (title of each file), recorded synthesis conditions
## 3. Methodologies
### 3.1 Experimental Design
- **Objective:** To characterize the structural and optical properties of functionalized metallic nanoparticles for phototherapy applications
- **Study Design:** Nanoparticles were synthesized under varying conditions and analyzed using spectroscopic and microscopic techniques
### 3.2 Data Collection Methods
- **Instruments Used:** UV-Vis spectrometer, Fourier Transform Infrared Spectroscopy (FTIR), Surface-Enhanced Raman spectrometer (SERS), Transmission Electron Microscope (TEM)
- **Measurement Parameters:** Absorption spectra (200-800 nm), Raman shift (cm-1), Wavelength (cm-1), nanoparticle size distribution (nm)
- **Calibration and Quality Control:** Spectrometers calibrated with reference standards, TEM imaging verified with literature data
## 4. Data Processing
### 4.1 Preprocessing
- **Raw Data Format:** CSV and TXT files for spectra, TIFF images for TEM, additional WXT files for SERS 
- **Data Cleaning Steps:** Removal of outliers, baseline correction for spectra
- **Normalization and Transformation:** Spectra normalized to peak intensity, background subtraction applied
### 4.2 Analysis Techniques

- **Statistical Methods:** Peak deconvolution and analyzing 
- **Software and Tools:** Origin Software for spectroscopic data, ImageJ for TEM image analysis
## 5. Data Files Description
- **File Formats:** CSV and TXT for spectral data, TIFF for microscopy images 
- **Naming Conventions:** `samplename_modification_ID.extension` (e.g., `Au_NPs_PEI_centri_1.txt`)
## 6. Reproducibility
- **Instructions for Reproducing Results:** Use Origin, Excel, PowerPoint or other type of available software to reproduce figures and analyses
- **Limitations and Considerations:** Variability in nanoparticle synthesis may affect reproducibility; data preprocessing methods should be consistently applied
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This README document ensures transparency and reproducibility of the research process by providing comprehensive details on the study's data and methodologies. Please refer to this file before using the dataset.

